Newly Generated Neurons at 2 Months Post-Status Epilepticus Are Functionally Integrated into Neuronal Circuitry in Mouse Hippocampus (Abbreviated title: Neurogenesis in Chronically Epileptic Hippocampus).

Newly Generated Neurons at 2 Months Post-Status Epilepticus Are Functionally Integrated into Neuronal Circuitry in Mouse Hippocampus (Abbreviated title: Neurogenesis in Chronically Epileptic Hippocampus).
Exp Neurol. 2015 Sep 15;
Authors: Hu M, Zhu K, Chen XL, Zhang YJ, Zhang JS, Xiao XL, Liu JX, Liu Y
Abstract
Emerging evidence has linked chronic temporal lobe epilepsy to dramatically reduced neurogenesis in the dentate gyrus. However, the profile of different components of neurogenesis in the chronically epileptic hippocampus is still unclear, especially the incorporation of newly generated cells. To address the issue, newly generated cells in the sub-granular zone of the dentate gyrus were labeled by the proliferation marker Bromodeoxyuridine (BrdU) or Retroviral Vector Expressing Green Fluorescent Protein 2months after pilocarpine-induced status epilepticus. The newly generated neurons that extended axons to CA3 area or integrated into memory circuits were visualized by cholera toxin B subunit retrograde tracing, and detecting activation of BrdU(+) cells following a recall of spatial memory test at the chronic stage of TLE. We found that the microenvironment was still able to sustain significant neuronal differentiation of newly generated cells at 2months post-status epilepticus time-point, and newly added neurons into granular cell layer were still able to integrate into neuronal circuitry, both anatomically and functionally. Qu...

Source: Experimental Neurology - September 15, 2015 Category: Neurology Authors: Hu M, Zhu K, Chen XL, Zhang YJ, Zhang JS, Xiao XL, Liu JX, Liu Y Tags: Exp Neurol Source Type: research