Simultaneous genotyping of four single nucleotide polymorphisms associated with risk factors of hemostasis disorders.

Simultaneous genotyping of four single nucleotide polymorphisms associated with risk factors of hemostasis disorders. Comb Chem High Throughput Screen. 2015 Sep 16; Authors: Bashmakova EE, Krasitskaya VV, Frank LA Abstract Multiplex simultaneous genotyping technique was developed for four polymorphisms in genes coding for blood coagulation factors and homocysteine metabolism which are considered as thrombophilia related mutations: FV Leiden, FII G20210A, MTHFR C677T, and FVII G10976A. It is based on primer extension reaction with the following bioluminescent solid-phase microassay. At that, two diļ¬€erent in bioluminescence obelin mutants were applied to simultaneous detection of two gene allelic variants. The assay is carried out in microtiter plate format and provides fast and reliable genotyping of four single nucleotide polymorphisms in four different genes within 2.5 hours. A large number of clinical samples were analyzed and the obtained results were found to be in complete correlation with those obtained by using conventional RT-PCR techniques. PMID: 26377543 [PubMed - as supplied by publisher]
Source: Combinatorial Chemistry and High Throughput Screening - Category: Chemistry Authors: Tags: Comb Chem High Throughput Screen Source Type: research

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