Persistent Change in Cardiac Fibroblast Physiology Following Transient ACE Inhibition.

Persistent Change in Cardiac Fibroblast Physiology Following Transient ACE Inhibition. Am J Physiol Heart Circ Physiol. 2015 Sep 14;:ajpheart.00615.2015 Authors: D'Souza KM, Biwer LA, Madhavpeddi L, Ramaiah P, Shahid W, Hale TM Abstract Transient angiotensin converting enzyme (ACE) inhibition induces persistent changes that protect against future nitric oxide synthase (NOS) inhibitor-induced cardiac fibrosis and inflammation. Given the role of fibroblasts in mediating these effects, the present study investigates whether prior ACE inhibition produced persistent changes in cardiac fibroblast physiology. Adult male spontaneously hypertensive rats (SHR) were treated with vehicle (C+L) or the ACE inhibitor, enalapril (E+L) for 2 weeks followed by a 2-week washout period, and a subsequent 7-day challenge with the NOS inhibitor, L-NAME. A third set of untreated SHR served as control. At the end of the study period, cardiac fibroblasts were isolated from control, C+L, and E+L left ventricles to assess proliferation rate, collagen expression, and chemokine release in vitro. After 7 days of NOS inhibition there were areas of myocardial injury, but no significant change in collagen deposition in E+L and C+L hearts in vivo. In vitro, cardiac fibroblasts isolated from C+L, but not E+L, were hyperproliferative, demonstrated increased collagen I gene expression, and an elevated secretion of the macrophage-recruiting chemokines, monocyte chemoattra...
Source: American Journal of Physiology. Heart and Circulatory Physiology - Category: Physiology Authors: Tags: Am J Physiol Heart Circ Physiol Source Type: research