High resolution melt curve analysis to confirm the presence of co-circulating isolates of Avian Nephritis Virus in commercial chicken flocks.

High resolution melt curve analysis to confirm the presence of co-circulating isolates of Avian Nephritis Virus in commercial chicken flocks. Avian Pathol. 2015 Sep 12;:1-30 Authors: Chamings A, Hewson KA, O'Rourke D, Ignjatovic J, Noormohammadi AH Abstract Avian Nephritis Virus (ANV) has been implicated in poor growth and renal disease of young chickens. This paper describes the development of a reverse-transcriptase polymerase chain reaction (RT-PCR) for the detection of ANV in commercial meat chickens and the use of High Resolution Melt (HRM) curves to detect the presence of genetically different ANVs. Pooled cloacal swabs from both healthy and ill commercial chicken broiler flocks were tested for the presence of ANV using a combination of PCR, molecular cloning, HRM curve analysis and sequencing. Except for one, all specimens were found to contain two genetically different ANVs. Phylogenetic analysis of the capsid amino acid sequences revealed the presence of four of six groups of ANV identified previously in other countries as well as two novel groups of ANV. Phylogenetic analysis of nucleotide sequences of partial polymerase, capsid and 3' untranslated regions reveal that the genes of individual ANV virus isolates have different ancestors. This was shown to be due to a template switching event in the capsid gene that resulted in the 3' end of the capsid gene and the 3' untranslated region of one ANV isolate being transferred to...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research