Bacterial CRISPR: accomplishments and prospects.

Bacterial CRISPR: accomplishments and prospects.
Curr Opin Microbiol. 2015 Sep 9;27:121-126
Authors: Peters JM, Silvis MR, Zhao D, Hawkins JS, Gross CA, Qi LS
Abstract
In this review we briefly describe the development of CRISPR tools for genome editing and control of transcription in bacteria. We focus on the Type II CRISPR/Cas9 system, provide specific examples for use of the system, and highlight the advantages and disadvantages of CRISPR versus other techniques. We suggest potential strategies for combining CRISPR tools with high-throughput approaches to elucidate gene function in bacteria.
PMID: 26363124 [PubMed - as supplied by publisher]

Source: Current Opinion in Microbiology - September 9, 2015 Category: Microbiology Authors: Peters JM, Silvis MR, Zhao D, Hawkins JS, Gross CA, Qi LS Tags: Curr Opin Microbiol Source Type: research