Haplotype Determination Using Allele-Specific Amplification Combined with Long-Range Real-Time PCR

Cytochrome P450 (CYP) 2D6, a major contributor to the metabolism and bioactivation of many clinically used drugs, is encoded by a complex, highly polymorphic gene locus. To aid in the characterization of CYP2D6 allelic variation, we developed allele-specific amplification combined with real-time long-range (ASXL)-PCR to amplify only the allele of interest for further characterization. This development was achieved utilizing single-nucleotide polymorphisms in the upstream region of CYP2D6 and a universal CYP2D6-specific reverse primer.

Source: Journal of Molecular Diagnostics - August 31, 2015 Category: Pathology Authors: Andrea Gaedigk, Amanda K. Riffel, J. Steven Leeder Tags: Regular Article Source Type: research