Genetic code expansion enabled site-specific dual-color protein labeling: superresolution microscopy and beyond.

Genetic code expansion enabled site-specific dual-color protein labeling: superresolution microscopy and beyond.
Curr Opin Chem Biol. 2015 Aug 21;28:164-173
Authors: Nikić I, Lemke EA
Abstract
Genetic code expansion is emerging as an important tool for manipulation and labeling of proteins in vitro and in vivo. In combination with click-chemistry it allows site-specific labeling of target proteins with small organic fluorophores. This is achieved by cotranslational incorporation of noncanonical amino acids (ncAAs) in target proteins via orthogonal tRNA/amino-acyl tRNA synthetase pairs. In a subsequent step, ncAAs are labeled with small dyes via click-chemistry. Small labeling tags and free choice of which fluorophore to use and where to put it into the protein are of particular importance for single molecule science and superresolution microscopy. Such genetically encoded click chemistry tools facilitate high resolution studies of protein function in live cells, viral imaging, as well as the improved design of antibody-drug conjugates.
PMID: 26302384 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - August 21, 2015 Category: Biochemistry Authors: Nikić I, Lemke EA Tags: Curr Opin Chem Biol Source Type: research