Protein-specific imaging of posttranslational modifications.

Protein-specific imaging of posttranslational modifications. Curr Opin Chem Biol. 2015 Aug 17;28:156-163 Authors: Lin W, Gao L, Chen X Abstract Protein posttranslational modifications (PTMs) modulate protein function, trafficking, and interactions. Many PTMs ubiquitously occurs on hundreds and thousands of proteins, which makes cellular imaging of the PTM state of a specific protein like looking for a needle in a haystack. A proximity-enabled strategy, which exploits the spatial proximity between the PTM and the modified protein, has emerged as a valuable tool for protein-specific imaging of PTMs in single cells and tissue sections. The protein and the PTM are dually labeled with two distinct tags, which enable the generation of the nanometer proximity-dependent fluorescent signals for visualization. Herein, we review recent advances in the methodological developments and the applications of the proximity-enabled protein-specific imaging in studying phosphorylation, glycosylation, and lipidation. PMID: 26291409 [PubMed - as supplied by publisher]
Source: Current Opinion in Chemical Biology - Category: Biochemistry Authors: Tags: Curr Opin Chem Biol Source Type: research