The Charms of the CHRM Receptors: Apoptotic and Amoebicidal effects of Dicyclomine on Acanthamoeba castellanii.
The Charms of the CHRM Receptors: Apoptotic and Amoebicidal effects of Dicyclomine on Acanthamoeba castellanii.
Antimicrob Agents Chemother. 2015 Aug 10;
Authors: Baig AM
Abstract
G-protein coupled receptors (GPCRs) are conserved amongst the eukaryotic cells are known to perform vital functions. Recently, anticholinergic drugs that target muscarinic cholinergic receptors have been found to kill pathogenic protists like Acanthamoeba, Balamuthia mandrillaris and Naegleria fowleri. Because of these effects, it was logical to expect a primeval cholinergic counterpart of this receptor on Acanthamoeba. Bioinformatics searches were made at data banks of Acanthamoeba genome and proteome. Immunostaining was done by using anti-human muscarinic M-1 antibody. Incubation assays were performed using Dicyclomine and Acetylcholine. The type of cell deaths observed with antagonist was analysed by staining and flow cytometry and correlated to the validated GPCR pathways on Acanthamoeba spp. In conducting Bioinformatics analysis, an identical protein homology for the cholinergic receptors was not found. We discovered few parallel hypothetical proteins, of which the ACA1_300890 shows the orthosteric binding amino acids residue positioning and the E2/i3 loop amino acid sequence homology of the cholinergic subtype receptor (CHRM1). The anti-human CHRM-1 antibody immunostaining and western blot illustrated the expression of an immunoreactive cell surface a...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Baig AM Tags: Antimicrob Agents Chemother Source Type: research