Genotyping Giardia intestinalis Using DNA Extracted from Long-Term-Preserved Human Specimens Stained with Chlorasol Black E.

Genotyping Giardia intestinalis Using DNA Extracted from Long-Term-Preserved Human Specimens Stained with Chlorasol Black E.
Jpn J Infect Dis. 2015 Aug 7;
Authors: Nishida Y, Morimoto N, Korenaga M, Komatsu Y, Takeuchi H, Matsumura Y, Sugiura T
Abstract
Giardia intestinalis is a parasitic protozoan that causes diarrhea and abdominal pain in humans. Studies on the Giardia genotypes are thought to be important for understanding their infection routes and prevalence. However, there are few reports on genotyping of the pathogen in human giardiasis in Japan. We genotyped G. intestinalis using DNA extracted from chlorasol black E stained fecal smears from patients. The triosephosphate isomerase gene was amplified from 21 (91.3%) of 23 human fecal samples. Twelve (52.2%) of the pathogens detected were of genotype A, and nine (39.1%) of genotype B. A restriction fragment length polymorphism analysis showed that all of genotype A found in the present study were of genotype AI which was assumed to be zoonotic. The source of Giardia infections was not clear in the present study. The patients' histories of traveling abroad seemed not to be associated with Giardia genotypes. Most of cases have been suggested to be acquired the infections sporadically and domestically.
PMID: 26255725 [PubMed - as supplied by publisher]

Source: Japanese Journal of Infectious Diseases - August 7, 2015 Category: Infectious Diseases Authors: Nishida Y, Morimoto N, Korenaga M, Komatsu Y, Takeuchi H, Matsumura Y, Sugiura T Tags: Jpn J Infect Dis Source Type: research