Cytolethal distending toxin gene-based multiplex PCR assay for Campylobacter jejuni, C. coli, C. fetus, C. upsaliensis, C. hyointestinalis and C. lari.

In this study, we constructed a cytolethal distending toxin (cdt) B subunit gene-based multiplex polymerase chain reaction (PCR) assay to simultaneously detect and differentiate Campylobacter jejuni, C. coli, C. fetus, C. upsaliensis, C. hyointestinalis and C. lari. The species-specific PCR products were obtained from all 38 C. jejuni, 39 C. coli, 12 C. fetus, 22 C. upsaliensis, 24 C. hyointestinalis and 7 C. lari strains tested. On the other hand, no specific PCR products were obtained from other campylobacters and bacterial species tested (totally 41 strains). The multiplex PCR assay developed in this study provides a valuable tool for detection and differentiation of major 6 Campylobacter species, which are associated with gastrointestinal diseases in human. PMID: 26255737 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research