Post-stroke transplantation of adult subventricular zone derived neural progenitor cells - a comprehensive analysis of cell delivery routes and their underlying mechanisms.

Post-stroke transplantation of adult subventricular zone derived neural progenitor cells - a comprehensive analysis of cell delivery routes and their underlying mechanisms. Exp Neurol. 2015 Aug 4; Authors: Doeppner TR, Kaltwasser B, Teli MK, Sanchez-Mendoza EH, Kilic E, Bähr M, Hermann DM Abstract With neuroprotective approaches having failed until recently, current focus on experimental stroke research has switched towards manipulation of post-ischemic neuroregeneration. Transplantation of subventricular zone (SVZ) derived neural progenitor cells (NPCs) is a promising strategy for promotion of neurological recovery. Yet, fundamental questions including the optimal cell delivery route still have to be addressed. Consequently, male C57BL6 mice were exposed to transient focal cerebral ischemia and allowed to survive for as long as 84days post-stroke. At six hours post-stroke, NPCs were grafted using six different cell delivery routes, i.e. intravenous, intraarterial, ipsilateral intrastriatal, contralateral intrastriatal, ipsilateral intraventricular and ipsilateral intracortical injection. Control mice received PBS only using the aforementioned delivery routes. Intralesional numbers of GFP(+) NPCs were high only after ipsilateral intrastriatal transplantation, whereas other injection paradigms only yielded comparatively small numbers of grafted cells. However, acute neuroprotection and improved functional outcome was observed after b...
Source: Experimental Neurology - Category: Neurology Authors: Tags: Exp Neurol Source Type: research