Clinical application of micronucleus test in exfoliated buccal cells: a systematic review and metanalysis

Publication date: Available online 5 August 2015 Source:Mutation Research/Reviews in Mutation Research Author(s): Claudia Bolognesi, Stefano Bonassi, Siegfried Knasmueller, Michael Fenech, Marco Bruzzone, Cecilia Lando, Marcello Ceppi The micronucleus assay in uncultured exfoliated buccal mucosa cells, involving minimally invasive sampling, was successfully applied to evaluate inhalation and local exposure to genotoxic agents, impact of nutrition and lifestyle factors. The potential use of the assay in clinics to monitor the development of local oral lesions and as an early biomarker for tumors and different chronic disorders was also investigated. A systematic review of the literature was carried out focusing on the clinical application of the assay. The literature search updated to January 2015 allowed to retrieve 42 eligible articles. Fifty three percent of investigations are related to oral, head and neck cancer, and premalignant oral diseases. Our analysis evidences a potential usefulness of the MN assay applied in buccal exfoliated cells in the prescreening and in the follow up of precancerous oral lesions. A significant excess of MN, in patients compared with matched controls was observed for subgroups of oral and neck cancer (meta-MR of 2.40,95% CI: 2.02-2.85) and leukoplakia (meta-MR 1.88, 95% CI: 1.51-2.35). The meta-analysis of studies available on other tumors (meta-MR 2.00; 95% CI:1.66-2.41) indicates that the MN frequency in buccal cells could ...
Source: Mutation Research Reviews in Mutation Research - Category: Genetics & Stem Cells Source Type: research