Oxidative DNA cleavage by Cu(II) complexes: Effect of periphery substituent groups.

Oxidative DNA cleavage by Cu(II) complexes: Effect of periphery substituent groups.
J Inorg Biochem. 2015 Jul 20;
Authors: Wang W, Lee YA, Kim G, Kim SK, Lee GY, Kim J, Kim Y, Park GJ, Kim C
Abstract
A series of structurally-related [Cu(R-benzyl-dipicolylamine)(NO3)2] complexes, where R=methoxy- (1), methyl- (2), H- (3), fluoro- (4), and nitro-group (5), were synthesized, and their activity on DNA cleavage was investigated by linear dichroism (LD) and electrophoresis. The addition of a benzyl group to the dipicolylamine ligand of the [Cu(dipicolylamine)(NO3)2] complex (A), i.e., the [Cu(benzyl-dipicolylamine)(NO3)2] complex (3), caused significant enhancement in the efficiency of oxidative cleavage of both super-coiled (sc) and double stranded (ds) DNA, as evidenced by the electrophoresis pattern and faster decrease in the LD intensity at 260nm. The efficiency in DNA cleavage was also altered with further modifications of the benzyl group by the introduction of various substituents at the para-position. The cleavage efficiency appeared to be the largest when the methyl group was attached. The order of efficiency in DNA cleavage was methyl>methoxy≈H>fluoro≈nitro group. When an electron-withdrawing group was introduced, the cleavage efficiency decreased remarkably. The reactive oxygen species involved in the cleavage process were the superoxide radical and singlet oxygen. A possible mechanism for this variation in the DNA cle...

Source: Journal of Inorganic Biochemistry - July 20, 2015 Category: Biochemistry Authors: Wang W, Lee YA, Kim G, Kim SK, Lee GY, Kim J, Kim Y, Park GJ, Kim C Tags: J Inorg Biochem Source Type: research