Overnourishment during lactation induces metabolic and haemodynamic heart impairment during adulthood

In this study, we examined the effects of postnatal overfeeding on heart energy homeostasis and cardiac haemodynamics in adult male Swiss mice. Mice were divided during the suckling period into four groups of control or overfed pups in combination with baseline or ischaemia/reperfusion treatments (control group baseline - CGBL, overfed group baseline - OGBL, control group ischaemia/reperfusion - CGIR, overfed group ischaemia/reperfusion - OGIR). We measured end diastolic pressure (EDP), heart contraction speed (Max dP/dt), relaxation speed (Min dP/dt), isovolumetric relaxation time (Tau) and frequency by beats per minute (BPM). During baseline and ischaemia/reperfusion, we studied key proteins such as AKT1, AKT2, AKT3, pAKT, AMPK, pAMPK, IRβ, PTP1B, IRS1, FABP, CD36, PI3K and PGC1α. We studied ANP, BNP, CPT1 and UCP3 expression as markers of cardiac hypertrophy and energetic metabolism. Cardiac fibrosis was analysed by quantification of collagen deposition which is increased in OGBL and OGIR compared to control groups. OGBL group showed EDP reduced compared to CGBL and high Max dp/dt compared to OGBL. Ischemia/reperfusion increased EDP and Min dp/dt in intragroup comparison. In contrast, Tau and frequency were not significantly different among groups. OGIR mice showed significant alterations in heart metabolism proteins, including AKT2, pAKT/AKT1, pAKT/AKT2, AMPK, pAMPK/AMPK, PTP1B, IRS1, FABP and CD36. Furthermore, ANP, BNP, CPT1 and UCP3 mRNA expression alterations indica...
Source: Nutrition, Metabolism and Cardiovascular Diseases - Category: Nutrition Source Type: research