Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil.

Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil.
Mem Inst Oswaldo Cruz. 2015 Jul 17;
Authors: Santos JJ, Magalhães T, Silva Junior JV, Silva AN, Cordeiro MT, Gil LH
Abstract
Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.
PMID: 26200712 [PubMed - as supplied by publisher]

Source: Memorias do Instituto Oswaldo Cruz - July 17, 2015 Category: Infectious Diseases Authors: Santos JJ, Magalhães T, Silva Junior JV, Silva AN, Cordeiro MT, Gil LH Tags: Mem Inst Oswaldo Cruz Source Type: research