Octadecaneuropeptide ODN prevents hydrogen peroxide-induced oxidative damage of biomolecules in cultured rat astrocytes

Publication date: Available online 2 July 2015 Source:Peptides Author(s): Yosra Hamdi , Hadhemi Kaddour , David Vaudry , Jérôme Leprince , Amira Zarrouk , Mohamed Hammami , Hubert Vaudry , Marie-Christine Tonon , Mohamed Amri , Olfa Masmoudi-Kouki Oxidative stress, associated with a variety of disorders including neurodegenerative diseases, is a major cause of cellular dysfunction and biomolecule damages which play a crucial role in neuronal apoptosis. Astrocytes specifically synthesize and release endozepines, a family of regulatory peptides, including the octadecaneuropeptide ODN. We have recently shown that ODN is a potent glioprotective agent that prevents hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis. The purpose of the present study was to investigate the potential protective effect of ODN on oxidative-generated damage of biomolecules in cultured rat astrocytes. Incubation of cells with subnanomolar concentrations of ODN (0.1 fM to 0.1 nM) inhibited H2O2-evoked reactive oxygen species accumulation and cell death in a concentration-dependent manner. Exposure of H2O2-treated cells to 0.1 nM ODN inhibited superoxide anion generation and blocked oxidative damage of cell molecules caused by H2O2 i.e. formation and accumulation of lipid oxidation products, malondialdehydes and conjugated dienes, and protein carbonyl compounds. Taken together, these data demonstrate for the first time that ODN prevents oxidative stress-induced alteration of ce...
Source: Peptides - Category: Biochemistry Source Type: research