Diet-induced pre-diabetes slows cardiac conductance and promotes arrhythmogenesis
This study describes electrophysiological alterations in a diet-induced pre-diabetic rat model and examines the underlying mechanism.
Methods:
Sprague–Dawley rats were fed either high-fat diet and fructose water or normal chow and water for 6 weeks. The electrophysiological properties of the whole heart was analyzed by in vivo surface ECG recordings, as wells as ex vivo in Langendorff perfused hearts during baseline, ischemia and re-perfussion. Conduction velocity was examined in isolated tissue strips. Ion channel and gap junction conductances were analyzed by patch-clamp studies in isolated cardiomyocytes. Fibrosis was examined by Masson’s Trichrome staining and thin-layer chromatography was used to analyze cardiac lipid content. Connexin43 (Cx43) expression and distribution was examined by western blotting and immunofluorescence respectively.
Results:
Following 6 weeks of feeding, fructose-fat fed rats (FFFRs) showed QRS prolongation compared to controls (16.1 ± 0.51 (n = 6) vs. 14.7 ± 0.32 ms (n = 4), p
Source: Cardiovascular Diabetology - Category: Cardiology Authors: Lene AxelsenKirstine CalloeThomas BraunsteinMads RiemannJohannes HofgaardBo LiangChrista JensenKristine OlsenEmil BartelsUlrik BaandrupThomas JespersenLars NielsenNiels-Henrik Holstein-RathlouMorten Nielsen Source Type: research
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