Oligonucleotide PIK3CA/Chromosome 3 Dual Hybridization Automated Assay with Improved Signals, One-Hour Hybridization, and No Use of Blocking DNA

The PIK3CA gene at chromosome 3q26.32 was found to be amplified in up to 45% of patients with squamous cell carcinoma of the lung. The strong correlation between PIK3CA amplification and increased phosphatidylinositol 3-kinase (PI3K) pathway activities suggested that PIK3CA gene copy number is a potential predictive biomarker for PI3K inhibitors. Currently, all microscopic assessments of PIK3CA and chromosome 3 (CHR3) copy numbers use fluorescence in situ hybridization. PIK3CA probes are derived from bacterial artificial chromosomes whereas CHR3 probes are derived mainly from the plasmid pHS05.

Source: Journal of Molecular Diagnostics - July 8, 2015 Category: Pathology Authors: Wenjun Zhang, Antony Hubbard, Leslie Baca-Parkinson, Stacey Stanislaw, Frank Vladich, Mark D. Robida, James G. Grille, Daniel Maxwell, Tsu-Shuen Tsao, William Carroll, Tracie Gardner, June Clements, Shalini Singh, Lei Tang Tags: Regular Article Source Type: research