Droplet Digital PCR for Absolute Quantification of  Gene Rearrangement in Lung Adenocarcinoma

Crizotinib treatment significantly prolongs progression-free survival, increases response rates, and improves the quality of life in patients with ALK-positive non–small-cell lung cancer. Droplet Digital PCR (ddPCR), a recently developed technique with high sensitivity and specificity, was used in this study to evaluate the association between the abundance of ALK rearrangements and crizotinib effectiveness. FFPE tissues were obtained from 103 consecutive patients with lung adenocarcinoma. Fluorescent in situ hybridization (FISH) and ddPCR were performed.

Source: Journal of Molecular Diagnostics - July 2, 2015 Category: Pathology Authors: Qiushi Wang, Xin Yang, Yong He, Qiang Ma, Li Lin, Ping Fu, Hualiang Xiao Tags: Regular Article Source Type: research