MicroRNA-150 deletion in mice protects kidney from myocardial infarction induced acute kidney injury.

MicroRNA-150 deletion in mice protects kidney from myocardial infarction induced acute kidney injury. Am J Physiol Renal Physiol. 2015 Jun 24;:ajprenal.00076.2015 Authors: Ranganathan P, Jayakumar C, Tang Y, Park KM, Teoh JP, Su H, Li J, Kim IM, Ramesh G Abstract Despite greater understanding of acute kidney injury (AKI) in animals models, many of the preclinical studies are not translatable. Most of the data were derived from a bilateral renal pedicle clamping model with warm ischemia. However, ischemic injury of the kidney in human is distinctly different and does not involve clamping of renal vessel. Using permanent ligation of the left anterior descending coronary artery model to test the role of miR-150 in acute kidney injury. Myocardial infarction in this model causes AKI which is similar to human cardiac bypass surgery. Moreover, the time course of serum creatinine and biomarker elevation were also similar to human ischemic injury. Deletion of miR-150 suppressed AKI which was associated with suppression of inflammation and interstitial cell apoptosis. Immunofluorescence staining with endothelial marker and marker of apoptosis suggested that dying cells are mostly endothelial cells with minimal epithelial cell apoptosis in this model. Interestingly, deletion of miR-150 also suppressed interstitial fibrosis. Consistent with protection, miR-150 deletion causes induction of its target gene IGF-1R and overexpression of miR-150 in e...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research