Extraction and Purification of Phlorotannins from Brown Algae

We describe here a simple procedure based on the use of water-organic solvent mixtures for the extraction of phlorotannins. Crude extracts are semi-purified and fractionated by separating methods based on both the polarity and the molecular size of compounds. Phenols are then quantified by the Folin-Ciocalteu method and their radical-scavenging activity is characterized using the DPPH test. All along the purification process of phenolic compounds, the efficiency of separation is assessed by 1H-NMR.

Source: Springer protocols feed by Plant Sciences - June 26, 2015 Category: Biology Source Type: news