Optimal extraction of polysaccharides from < em > Stevia rebaudiana < /em > roots for protection against hydrogen peroxide-induced oxidative damage in RAW264.7 cells

In this study, we optimised the extraction of a polysaccharide (SRRP) from S. rebaudiana roots by employing a Box-Behnken design and response surface methodology. The optimal extraction conditions were as follows: 93.57 min, 71.67 °C, and a water-to-raw material ratio of 21.40 mL/g. Under these conditions, 14.00 ± 0.35% of crude polysaccharide was obtained. Treatment of RAW264.7 cells with SRRP prior to the addition of H2O2, a major contributor to oxidative damage, significantly increased cell viability. In addition, SRRP increased the levels of superoxide dismutase, catalase, and glutathione and reduced the levels of malondialdehyde in RAW264.7 cells. Therefore, SRRP can provide effective protection against H2O2-induced oxidative damage. These findings indicate the potential of SRRP as a natural antioxidant in the food and pharmaceutical industries.PMID:37791599 | DOI:10.1080/14786419.2023.2263905
Source: Natural Product Research - Category: Biochemistry Authors: Source Type: research