Simple two-step purification and characterisation of peroxidase from < em > Citrullus colocynthis < /em >

Nat Prod Res. 2023 Aug 25:1-10. doi: 10.1080/14786419.2023.2248644. Online ahead of print.ABSTRACTPeroxidase is a biotechnologically important enzyme. The purification of peroxidase from the root of Citrullus colocynthis was carried out in a simple two-step process with maximum purity level. The sample was extracted in a high salt buffer, and the enzyme was partially purified with a Q-Sepharose anion exchange column. Final purification was carried out with HighLoad 16/600 Superdex G-75 column. The purified protein was analysed with SDS gel electrophoresis, which suggested a single band of approximately 35 kDa. Further, the enzyme was identified with the help of Mass spectrometric analysis using an ESI-QTOF Mass spectrometer. The study will be helpful for the isolation and its commercial uses in biotechnology.PMID:37621192 | DOI:10.1080/14786419.2023.2248644
Source: Natural Product Research - Category: Biochemistry Authors: Source Type: research