Peroxynitrite in Sarcoidosis: Relation to Mycobacterium Stationary Phase.

In this study, therefore, we evaluated NOx and ONOO(-) levels in supernatants of peripheral blood mononuclear cells cultures treated with Mtb-HSPs from 20 SA patients, 19 TB patients, and 21 healthy volunteers using Griess and rhodamine fluorescence methods. We found significantly greater NOx and ONOO(-) concentrations with/without Mtb-HSPs stimulation in SA and TB patients than in controls. However, there were significantly lower NOx and higher ONOO(-) levels after Mtb-HSPs induction in SA than TB patients. In summary, in contrast to active TB, increased ONOO(-) concentration may explain the low level of NOx with induction of M. tuberculosis genetic dormancy program via higher Mtb-HSP16 expression in SA. PMID: 26017730 [PubMed - as supplied by publisher]
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research