Expression of HIF-1A/VEGF/ING-4 Axis in Pulmonary Sarcoidosis.

Expression of HIF-1A/VEGF/ING-4 Axis in Pulmonary Sarcoidosis. Adv Exp Med Biol. 2015 May 29; Authors: Piotrowski WJ, Kiszałkiewicz J, Pastuszak-Lewandoska D, Górski P, Antczak A, Migdalska-Sęk M, Górski W, Czarnecka KH, Domańska D, Nawrot E, Brzeziańska-Lasota E Abstract Angiogenesis/angiostasis regulated by hypoxia inducible factor-1A (HIF-1A)/vascular endothelial growth factor (VEGF)/inhibitor of growth protein 4 (ING-4) axis may be crucial for the course and outcome of sarcoidosis. Overexpression of angiogenic factors (activation of VEGF through HIF-1A) may predispose to chronic course and lung fibrosis, whereas immunoangiostasis (related to an overexpression of inhibitory ING-4) may be involved in granuloma formation in early sarcoid inflammation, or sustained or recurrent formation of granulomas. In this work we investigated gene expression of HIF-1A, VEGF and ING-4 in bronchoalveolar fluid (BALF) cells and in peripheral blood (PB) lymphocytes of sarcoidosis patients (n = 94), to better understand mechanisms of the disease and to search for its biomarkers. The relative gene expression level (RQ value) was analyzed by qPCR. The results were evaluated according to the presence of lung parenchymal involvement (radiological stage I vs. II-IV), acute vs. insidious onset, lung function tests, calcium metabolism parameters, percentage of lymphocytes (BALL %) and BAL CD4(+)/CD8(+) in BALF, age, and gender. In BALF cells, the ING-...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research