Loss of α(E)-catenin promotes Fas mediated apoptosis in tubular epithelial cells

In this study, cells were challenged with nephrotoxicant cisplatin to induce AKI. A ~5.5-fold increase in Fas expression in C2 (stable α(E)-catenin knockdown) relative to NT3 (non-targeted control) cells was seen. Increased caspase-8 and -9 activation was induced by cisplatin in C2 as compared to NT3 cells. In addition, decreased Bcl-2 expression and increased BID cleavage and cytochrome C release were detected in C2 cells after cisplatin challenge. Treating the cells with cisplatin, in combination with a Bcl-2 inhibitor, decreased the viability of NT3 cells to the same level as C2 cells after cisplatin. Furthermore, caspase-3/-7 activation is blocked by Fas, caspase-8, caspase-9 and pan-caspase inhibitors. These inhibitors also completely abolished the difference in viability between NT3 and C2 cells in response to cisplatin. These results demonstrate a Fas-mediated apoptotic signaling pathway that is enhanced by the age-dependent loss of α(E)-catenin in renal tubule epithelial cells.
Source: Apoptosis - Category: Molecular Biology Source Type: research

Related Links:

Publication date: Available online 29 May 2020Source: Plant ScienceAuthor(s): Tingting Bian, Yan Ma, Jing Guo, Yang Wu, Dongmei Shi, Xianfeng Guo
Source: Plant Science - Category: Biology Source Type: research
Publication date: Available online 30 May 2020Source: Redox BiologyAuthor(s): Desong Kong, Zili Zhang, Liping Chen, Weifang Huang, Feng Zhang, Ling Wang, Yu Wang, Peng Cao, Shizhong Zheng
Source: Redox Biology - Category: Biology Source Type: research
Publication date: December 2020Source: NJAS - Wageningen Journal of Life Sciences, Volume 92Author(s): Le Thi Hoa Sen, Jennifer Bond, Alexandra Winkels, Nguyen Hoang Khanh Linh, Nguyen Tien Dung
Source: NJAS Wageningen Journal of Life Sciences - Category: Biology Source Type: research
Publication date: Available online 30 May 2020Source: Life SciencesAuthor(s): Qi Fu, Zhenhai Yu
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 30 May 2020Source: Life SciencesAuthor(s): Lakshmikripa Jagannathan, Emily Socks, Priya Balasubramanian, Robert McGowan, Thomas M. Herdt, Reza Kianian, Sheba M.J. MohanKumar, P.S. MohanKumar
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 29 May 2020Source: Life SciencesAuthor(s): Xiaoping Pan, Chen Wang, Yan Li, Lida Zhu, Ti Zhang
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 29 May 2020Source: Life SciencesAuthor(s): Nagesh Kishan Panchal, E.P. Sabina
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 29 May 2020Source: Life SciencesAuthor(s): Luping Wang, Lu Yun, Xiaojun Wang, Liying Sha, Luning Wang, Yingying Sui, Hui Zhang
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 29 May 2020Source: Medical Mycology Case ReportsAuthor(s): Eszter Szlávicz, Csongor Németh, Éva Szepes, Csaba Gyömörei, Rolland Gyulai, Zsuzsanna Lengyel
Source: Medical Mycology Case Reports - Category: Biology Source Type: research
Publication date: Available online 19 May 2020Source: Journal de Mycologie MédicaleAuthor(s): Jordan Leroy, Fanny Vuotto, Vicky Le, Marjorie Cornu, Nadine François, Laure Marceau, Clémence Fichet, Séverine Loridant, Boualem Sendid
Source: Journal of Medical Mycology - Category: Biology Source Type: research
More News: Molecular Biology | Study | Urology & Nephrology