Differential expression of the calcium-sensing receptor in the ischemic and border zones after transient focal cerebral ischemia in rats

Publication date: Available online 23 May 2015 Source:Journal of Chemical Neuroanatomy Author(s): Jeong Sook Noh , Ha-Jin Pak , Yoo-Jin Shin , Tae-Ryong Riew , Joo-Hee Park , Young Wha Moon , Mun-Yong Lee G-protein-coupled calcium-sensing receptor (CaSR) has been recently recognized as an important modulator of diverse cellular functions, beyond the regulation of systemic calcium homeostasis. To identify whether CaSR is involved in the pathophysiology of stroke, we studied the spatiotemporal regulation of CaSR protein expression in rats undergoing transient focal cerebral ischemia, which was induced by middle cerebral artery occlusion. We observed very weak or negligible immunoreactivity for CaSR in the striatum of sham-operated rats, as well as in the contralateral striatum of ischemic rats after reperfusion. However, CaSR expression was induced in the ischemic and border zones of the lesion in ischemic rats. Six hours post-reperfusion there was an upregulation of CaSR in the ischemic zone, which seemed to decrease after seven days. This upregulation preferentially affected some neurons and cells associated with blood vessels, particularly endothelial cells and pericytes. In contrast, CaSR expression in the peri-infarct region was prominent three days after reperfusion, and with the exception of some neurons, it was mostly located in reactive astrocytes, up to day 14 after ischemia. On the other hand, activated microglia/macrophages in both the ischemic and border...
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research