JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay for the detection of genotoxic carcinogens: I. Summary of pre-validation study results

Publication date: Available online 5 May 2015 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Yoshifumi Uno , Hajime Kojima , Takashi Omori , Raffaella Corvi , Masamistu Honma , Leonard M. Schechtman , Raymond R. Tice , Brian Burlinson , Patricia A. Escobar , Andrew R. Kraynak , Yuzuki Nakagawa , Madoka Nakajim , Kamala Pant , Norihide Asano , David Lovell , Takeshi Morita , Yasuo Ohno , Makoto Hayashi The in vivo rodent alkaline comet assay (comet assay) is used internationally to investigate the in vivo genotoxic potential of test chemicals. This assay, however, has not previously been formally validated. The Japanese Center for the Validation of Alternative Methods (JaCVAM), with the cooperation of the U.S. NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM)/the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the European Centre for the Validation of Alternative Methods (ECVAM), and the Japanese Environmental Mutagen Society/Mammalian Mutagenesis Study Group (JEMS/MMS), organized an international validation study to evaluate the reliability and relevance of the assay for identifying genotoxic carcinogens, using liver and stomach as target organs. The ultimate goal of this validation effort was to establish an Organization for Economic Co-operation and Development (OECD) test guideline. The purpose of the pre-validation studies (i.e., Phase 1...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research