Genotoxicity evaluation of benzene, di(2-ethylhexyl) phthalate, and trisodium ethylenediamine tetraacetic acid monohydrate using a combined rat comet/micronucleus assays

Publication date: Available online 8 May 2015 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Sachiko Kitamoto , Ryoko Matsuyama , Yasuaki Uematsu , Keiko Ogata , Mika Ota , Toru Yamada , Kaori Miyata , Juki Kimura , Hitoshi Funabashi , Koichi Saito As a part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo alkaline comet assay (comet assay), we examined DNA damage in the liver, stomach, and bone marrow of rats dosed orally three times with up to 2000mg/kg of benzene, di(2-ethylhexyl) phthalate, and trisodium ethylenediamine tetraacetic acid monohydrate. All three compounds gave negative results in the liver and stomach. In addition, a bone marrow comet and micronucleus analysis revealed that benzene, but not di(2-ethylhexyl) phthalate or trisodium ethylenediamine tetraacetic acid monohydrate induced a significant increase in the median % tail DNA and micronucleated polychromatic erythrocytes, compared with the respective concurrent vehicle control. These results were in good agreement with the previously reported genotoxicity findings for each compound. The present study has shown that combining the micronucleus test with the comet assay and carrying out these analyses simultaneously is effective in clarifying the mechanism of action of genotoxic compounds such as benzene.

Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - May 19, 2015 Category: Genetics & Stem Cells Source Type: research