Analytical Performance of a 15-Gene Prognostic Assay for Early-Stage Non–Small-Cell Lung Carcinoma Using RNA-Stabilized Tissue

A 15-gene prognostic signature for early-stage, completely resected, non–small-cell lung carcinoma, that distinguishes between patients with good and poor prognoses was clinically validated in prior studies. To achieve operational efficiencies, this study was designed to evaluate the assay's performance in RNA-stabilized tissue as an alternative to the fresh-frozen tissue format, originally used to develop the assay. The percent concordance between matched tissue formats was 84% (95% Wilson CI, 70%–92%), a level of agreement comparable to the inherent reproducibility of the assay observed within biological replicates of fresh-frozen tissue.

http://jmd.amjpathol.org/article/S1525-1578(15)00077-X/abstract?rss=yes

Source: Journal of Molecular Diagnostics - May 7, 2015 Category: Pathology Authors: Shuguang Huang, Nicholas J. Reitze, Amy L. Ewing, Suzanne McCreary, Arlette H. Uihlein, Stacey L. Brower, Dakun Wang, Tianhua Wang, Michael J. Gabrin, Katherine E. Keating, Jude Mulligan, Claire Wilson, Timothy Davison, Stuart McKenzie, Ming-Sound Tsao, F Tags: Regular Article Source Type: research