Antioxidant enzymes gene polymorphisms and hepatocellular carcinoma in hepatitis C virus-infected Egyptian patients

Abstract Oxidative stress is an imbalance between production and elimination of reactive metabolites of oxygen and nitrogen, in favor of their production leading to potential damage. During oxidative stress, biologically important molecules and cells can be damaged, and this can be significant in the pathogenesis of many diseases. Reactive oxygen species (ROS) and ROS-induced cytokines are known to trigger the apoptosis of some hepatocytes and therefore contribute to inflammation, regeneration, fibrogenesis, and carcinogenesis. The enzymes generally considered to be the frontline defense against ROS are catalase (CAT), the mitochondrial manganese superoxide dismutase (MnSOD), and glutathione peroxidase (GPX). Our aim in this work is to assess the possible association of the antioxidant enzyme polymorphisms of CAT (c.C-262T, rs1001179), MnSOD (p.Val16Ala, rs4880), and GPX1 (p.Pro 198 Leu, rs1050450) with the development of hepatocellular carcinoma (HCC) in a sample of hepatitis C virus (HCV)-infected Egyptian patients. Genetic polymorphisms were estimated in 40 HCC patients on top of HCV infection, 20 cirrhotic patients on top of HCV infection, and 20 healthy control individuals. Genetic polymorphisms of CAT (c.C-262T), MnSOD (p.Val16Ala), and GPX1 (p.Pro 198 Leu) were studied using PCR-RFLP technique. With regard to CAT enzyme polymorphism, the frequency of the CC was significantly higher in HCC and cirrhosis patients compared to the healthy control group ...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research