Inhibitory effects of nitrite on the reactions of bovine carbonic anhydrase II with CO2 and bicarbonate consistent with zinc-bound nitrite.

Inhibitory effects of nitrite on the reactions of bovine carbonic anhydrase II with CO2 and bicarbonate consistent with zinc-bound nitrite.
J Inorg Biochem. 2015 Apr 27;149:6-11
Authors: Nielsen PM, Fago A
Abstract
Carbonic anhydrase (CA) is a zinc enzyme that catalyzes hydration of carbon dioxide (CO2) and dehydration of bicarbonate in red blood cells, thus facilitating CO2 transport and excretion. Bovine CA II may also react with nitrite to generate nitric oxide, although nitrite is a known inhibitor of the CO2 hydration reaction. To address the potential in vivo interference of these reactions and the nature of nitrite binding to the enzyme, we here investigate the inhibitory effect of 10-30mM nitrite on Michaelis-Menten kinetics of CO2 hydration and bicarbonate dehydration by stopped-flow spectroscopy. Our data show that nitrite significantly affects the apparent dissociation constant KM for CO2 (11mM) and bicarbonate (221mM), and the turnover number kcat for the CO2 hydration (1.467×10(6)s(-1)) but not for the bicarbonate dehydration (7.927×10(5)s(-1)). These effects demonstrate mixed and competitive inhibition for the reaction with CO2 and bicarbonate, respectively, and are consistent with nitrite binding to the active site zinc. The high apparent dissociation constant found here for CO2, bicarbonate and nitrite (16-120mM) are all overall consistent with published data and reveal a large capacity of free enzyme available for ...

Source: Journal of Inorganic Biochemistry - April 27, 2015 Category: Biochemistry Authors: Nielsen PM, Fago A Tags: J Inorg Biochem Source Type: research