Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spacial learning and memory in mouse middle cerebral artery occlusion model

IntroductionGrowing evidence has brought stem cell therapy to the forefront as new promising approaches towards stroke treatment. Of all candidate seeding cells, adipose-derived stem cells (ADSCs) are considered as one of the most appropriate for stroke treatment. However, previous experimental data could not reach to an agreement on the efficacy of ADSC transplantation for treating stroke in vivo as well as its mechanism which hinders their further clinical translational application. Methods: To explore their in vivo mechanism of hADSC administration on neurological injury, hADSC were labeled with EGFP expressing FG12 lentivirus and injected into MCAO mouse infarct area by in situ way. Neurological function was evaluated by Rogers Scaling System and their spacial learning and memory was determined by Morris Test. TTC was carried out to compare the infarct area among groups. Histoimmunostaining was used to track the injected hADSCs for their in vivo migration, transdifferentiation and integration with the endogenous neuronal circuitry. To better address the underlying rescuing mechanism, qRT-PCR was performed on neural markers of MBP, MAP2, GFAP, microglia marker of Iba1. Results: It was found that hADSCs could promote both spacial learning and memory of MCAO mice. Co-localization of GFP and MAP2 were found in the whole cortex with significantly (P 
Source: BioMed Central - Category: Journals (General) Authors: Source Type: research