Microbial dynamics with CRC progression: a study of the mucosal microbiota at multiple sites in cancers, adenomatous polyps, and healthy controls

AbstractAccumulating evidence has related the gut microbiota to colorectal cancer (CRC).Fusobacterium nucleatum has repeatedly been linked to colorectal tumorigenesis. The aim of this study was to investigate microbial composition in different sampling sites, in order to profile the microbial dynamics with CRC progression. Further, we characterized the tumor-associatedF. nucleatum subspecies. Here, we conducted Illumina Miseq next-generation sequencing of the 16S rRNA V4 region in biopsy samples, to investigate microbiota alterations in cancer patients, patients with adenomatous polyp, and healthy controls in Norway. Further,Fusobacterium positive tumor biopsies were subjected to MinION nanopore sequencing ofFusobacterium-specific amplicons to characterize theFusobacterium species and subspecies. We found enrichment of oral biofilm-associated bacteria,Fusobacterium,Gemella,Parvimonas,Granulicatella,Leptotrichia,Peptostreptococcus,Campylobacter,Selenomonas,Porphyromonas, andPrevotella in cancer patients compared to adenomatous polyp patients and control patients. Higher abundance of amplicon sequence variants (ASVs) classified asPhascolarctobacterium,Bacteroides vulgatus,Bacteroides plebeius,Bacteroides eggerthii,Tyzzerella,Desulfovibrio,Frisingicoccus,Eubacterium coprostanoligenes  group, andLachnospiraceae were identified in cancer and adenomatous polyp patients compared to healthy controls.F. nucleatum ssp.animalis was the dominating subspecies.F. nucleatum ssp.nucleatum,F...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research