Podocyte hypertrophy precedes apoptosis under experimental diabetic conditions

Abstract Podocyte hypertrophy and apoptosis are two hallmarks of diabetic glomeruli, but the sequence in which these processes occur remains a matter of debate. Here we investigated the effects of inhibiting hypertrophy on apoptosis, and vice versa, in both podocytes and glomeruli, under diabetic conditions. Hypertrophy and apoptosis were inhibited using an epidermal growth factor receptor inhibitor (PKI 166) and a pan-caspase inhibitor (zAsp-DCB), respectively. We observed significant increases in the protein expression of p27, p21, phospho-eukaryotic elongation factor 4E-binding protein 1, and phospho-p70 S6 ribosomal protein kinase, in both cultured podocytes exposed to high-glucose (HG) medium, and streptozotocin-induced diabetes mellitus (DM) rat glomeruli. These increases were significantly inhibited by PKI 166, but not by zAsp-DCB. In addition, the amount of protein per cell, the relative cell size, and the glomerular volume were all significantly increased under diabetic conditions, and these changes were also blocked by treatment with PKI 166, but not zAsp-DCB. Increased protein expression of cleaved caspase-3 and cleaved poly (ADP-ribose) polymerase, together with increased Bax/Bcl-2 ratios, were also observed in HG-stimulated podocytes and DM glomeruli. Treatment with either zAsp-DCB or PKI 166 resulted in a significant attenuation of these effects. Both PKI 166 and zAsp-DCB also inhibited the increase in number of apoptotic cells, as assessed by Hoechst 3...
Source: Apoptosis - Category: Molecular Biology Source Type: research

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CONCLUSION: In hypertensive and diabetic patients, higher OPG values were associated with impaired LA function assessed by 2D-STE. In this high-risk patient group, serum OPG can be used as a risk predictor for LA mechanical dysfunction. PMID: 32462219 [PubMed - as supplied by publisher]
Source: Herz - Category: Cardiology Tags: Herz Source Type: research
Publication date: Available online 28 May 2020Source: Diabetes &Metabolic Syndrome: Clinical Research &ReviewsAuthor(s): Dukhabandhu Naik, Felix K. Jebasingh, Nihal Thomas, Sreekanth Raveendran, Samuel C. Raj Pallapati, JohnJude Prakash, S. Gowri, Binu Prathap Thomas
Source: Diabetes and Metabolic Syndrome: Clinical Research and Reviews - Category: Endocrinology Source Type: research
Omnipod DASH Insulin Management System (mmol/L configuration), Catalog Number PT000011 M/D: INT1D001MM - Product Usage: is intended for subcutaneous delivery of insulin at set and variable rates for the management of diabetes mellitus in persons requiring insulin.
Source: Medical Device Recalls - Category: Medical Devices Source Type: alerts
Fatal Strongyloides stercoralis hyperinfection syndrome in an alcoholic diabetic patient from México. Biomedica. 2020 May 01;40(Supl. 1):32-36 Authors: Rodríguez-Pérez EG, Arce-Mendoza AY, Saldívar-Palacios R, Escandón-Vargas K Abstract Strongyloides stercoralis hyperinfection syndrome is a medical emergency that requires a high level of suspicion. Immunocompromised patients are at high risk of hyperinfection syndrome; however, malnutrition, alcoholism, and diabetes mellitus also need to be considered as predisposing factors. The diagnosis and treatment of Strongyloid...
Source: Biomedica : Revista del Instituto Nacional de Salud - Category: International Medicine & Public Health Tags: Biomedica Source Type: research
Condition:   Sarcopenia Intervention:   Radiation: WHOLE BODY DXA Sponsors:   Dionyssiotis, Yannis, M.D.;   University of Thessaly Completed
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Gestational Diabetes Interventions:   Drug: Metformin;   Drug: Ursodeoxycholic Acid Sponsors:   King's College London;   Guy's and St Thomas' NHS Foundation Trust Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
ABSTRACT The coronavirus disease 2019 (covid-19) pandemic has caused a public health emergency worldwide. Risk, severity and mortality of the disease have been associated with non-communicable chronic diseases, such as diabetes mellitus. Accumulated evidence has caused great concern in countries with high prevalence of this morbidity, such as Brazil. This text shows the picture of diabetes in Brazil, followed by epidemiological data and explanatory hypothesis for the association between diabetes and covid-19. We emphasized how the burden of these two morbidities in a middle-income country has aggravated this pandemic scena...
Source: Revista de Saude Publica - Category: International Medicine & Public Health Source Type: research
Condition:   Sarcopenia Intervention:   Radiation: WHOLE BODY DXA Sponsors:   Dionyssiotis, Yannis, M.D.;   University of Thessaly Completed
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Gestational Diabetes Interventions:   Drug: Metformin;   Drug: Ursodeoxycholic Acid Sponsors:   King's College London;   Guy's and St Thomas' NHS Foundation Trust Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conclusions: There is shortage of data from prospective studies which could confirm the benefits of early treatment initiation based on the presence of the subclinical organ damage in T1DM. Most evidence comes from T2DM trials, where effective preventive measures were identified i.e.: smoking cessation, reasonable blood glucose control, efficacious hypertension treatment, and dyslipidemia management, as well as renoprotection. There is still a field for further research to see if routine assessment of asymptomatic vascular damage and early implementation of aggressive treatment would reduce mortality excess from CVD in T1D...
Source: Blood Pressure - Category: Hematology Tags: Blood Press Source Type: research
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