The Meteorin-like cytokine is upregulated in grass carp after infection with Aeromonas hydrophila

In this study, monoclonal antibodies were prepared against recombinant grass carp (Ctenopharyngodon idella, Ci) Metrnl-a in mice and characterized by Western blotting, flow cytometry and immunofluorescent microscopy. In grass carp infected with Aeromonus hydrophila (A. hydrophila), the cells expressing CiMetrnl-a markedly increased in the gills, head kidney and intestine. In the inflamed intestine caused by A. hydrophila infection, the CiMetrnl-a producing cells were detected mainly in the mucosal layer of anterior, middle and posterior segments. Consistently, qRT-PCR analysis showed that the mRNA expression of CiMetrnl-a was markedly induced. Our results suggest that CiMetrnl-a is involved in regulating intestine inflammation caused by bacterial infection.PMID:36608897 | DOI:10.1016/j.dci.2023.104632

Source: Developmental and Comparative Immunology - January 7, 2023 Category: Allergy & Immunology Authors: Zhao Jia Jianhua Feng Gaoliang Yuan Hehe Xiao Huifeng Dang Yanwei Zhang Kangyong Chen Jun Zou Junya Wang Source Type: research