Evaluation of the Real-Q V600E Detection Assay in Fine-Needle Aspiration Samples of Thyroid Nodules

Recently, several molecular assays for detecting the BRAF V600E mutation in fine-needle aspiration (FNA) specimens have been developed. Herein, we tested 294 consecutive FNA samples from patients with thyroid nodules with the Real-Q BRAF V600E detection assay (Real-Q). These results were compared with an allele-specific PCR-based kit using dual-priming oligonucleotides (AS-PCR). Any discordant results between the two tests were also analyzed by mutant enrichment with 3′-modified oligonucleotide sequencing.

Source: Journal of Molecular Diagnostics - April 29, 2015 Category: Pathology Authors: Kyung S. Park, Young L. Oh, Chang-Seok Ki, Jong-Won Kim Tags: Regular Article Source Type: research