Coexistence of tet(A) and blaKPC-2 in the ST11 hypervirulent tigecycline- and carbapenem-resistant Klebsiella pneumoniae isolated from a blood sample

This study aimed to characterize a hypervirulent tigecycline-resistant and carbapenem-resistantKlebsiella pneumoniae strain, XJ-K2, collected from a patient ’s blood. We tested antimicrobial susceptibility, virulence, and whole-genome sequencing (WGS) on strain XJ-K2. WGS data were used to identify virulence and resistance genes and to perform multilocus sequence typing (MLST) and phylogenetic analysis. Three novel plasmids, including a pLVPK-like vir ulence plasmid (pXJ-K2-p1) and two multiple resistance plasmids (pXJ-K2-KPC-2 and pXJ-K2-p3), were discovered in strain XJ-K2. The IncFII(pCRY) plasmid pXJ-K2-p3 carried thedfrA14,sul2,qnrS1,blaLAP-2, andtet(A) resistance genes. The IncFII(pHN7A8)/IncR plasmid pXJ-K2-KPC-2 also carried a range of resistance elements, containingrmtB,blaKPC-2,blaTEM-1,blaCTX-M-65, andfosA3. MLST analysis revealed that strain XJ-K2 belonged to sequence type 11 (ST11). Seven complete phage sequences and many virulence genes were found in strain XJ-K2. Meanwhile, antimicrobial susceptibility tests andG. mellonella larval infection models confirmed the extensively drug resistance (XDR) and hypervirulence of KJ-K2. To our knowledge, this is the first observation and description of the ST11 hypervirulent tigecycline- and carbapenem-resistantK. pneumoniae strain co-carryingblaKPC-2 and thetet(A) in a patient ’s blood in China. Further investigation is needed to understand the resistance and virulence mechanisms of this significant hypervirulent tigec...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research