Urinary Vimentin mRNA as a Potential Novel Biomarker of Renal Fibrosis.

Urinary Vimentin mRNA as a Potential Novel Biomarker of Renal Fibrosis. Am J Physiol Renal Physiol. 2015 Apr 22;:ajprenal.00449.2014 Authors: Cao YH, Lv LL, Zhang X, Hu H, Ding LH, Yin D, Zhang YZ, Ni HF, Chen PS, Liu BC Abstract Renal fibrosis is a histological outcome of chronic kidney disease (CKD) progression. However, the non-invasive detection of renal fibrosis remains a challenge. Here we constructed a renal fibrosis target mRNA array and used it to detect urinary mRNAs of CKD patients for investigating potential non-invasive biomarkers of renal fibrosis. We collected urine samples from 39 biopsy-proven CKD patients and 11 healthy controls in the training set. Urinary mRNA profiles of 86 genes showed a total of 22 mRNAs that were differentially expressed between CKD patients and controls (P<0.05), and vimentin (VIM) mRNA demonstrated the highest change-fold of 9.99 in CKD versus controls with robust correlations with decline of renal function and severity of tubulointerstitial fibrosis (TIF). Additionally, VIM mRNA further differentiated patients with moderate-to-severe fibrosis from none-to-mild fibrosis group with an AUC of 0.796 (P=0.008). A verification of VIM mRNA in the urine of an additional 96 patients and 20 controls showed that VIM is not only well correlated with renal function parameters, but also correlated with proteinuria, and renal fibrosis scores. Multiple logistic regression and ROC analysis further showed...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research