Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP

The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.

Source: Acta Crystallographica Section F - April 21, 2015 Category: Biochemistry Authors: Moen, S.O.Fairman, J.W.Barnes, S.R.Sullivan, A.Nakazawa-Hewitt, S.Van Voorhis, W.C.Staker, B.L.Lorimer, D.D.Myler, P.J.Edwards, T.E. Tags: prostaglandin infectious diseases leishmaniasis Chagas disease SSGCID research communications Source Type: research