Fe(2+) and Fe(3+) in micromolar concentrations modulate glycine-induced Cl(-) current in rat hippocampal neurons.

Fe(2+) and Fe(3+) in micromolar concentrations modulate glycine-induced Cl(-) current in rat hippocampal neurons.
Brain Res Bull. 2015 Apr 15;
Authors: Solntseva EI, Bukanova JV, Kondratenko RV, Skrebitsky VG
Abstract
The effects of Fe(2+) and Fe(3+) on glycine-activated chloride current (IGly) were studied in rat isolated pyramidal hippocampal neurons using patch-clamp technique in whole-cell configuration. 25, 100 or 500μM glycine were applied for 600ms with 40 s intervals. Fe(2+) and Fe(3+) were co-applied with glycine in the range of concentrations of 0.01-100μM. We found that Fe(2+) and Fe(3+) affected IGly in a similar manner. Two types of effects of iron on IGly were observed. In low concentrations (0.1μM) Fe ions caused an acceleration of the IGly desensitization, and the effect was more pronounced for IGly induced by 100 and 500μM glycine than by 25μM glycine. Higher Fe concentrations (1-100μM) decreased the peak amplitude of IGly with weak influence on its kinetics. The values of IC50 of the effect were close to 10μM for all glycine concentrations tested. The effect of iron on IGly peak did not depend on the membrane potential. This inhibition was noncompetitive and voltage-independent, suggesting that Fe ions do not exert their action on the agonist binding site of GlyRs or block the channel pore. An important characteristic of both effects of Fe was their progressive development during repetitive Fe applications (u...

Source: Brain Research Bulletin - April 15, 2015 Category: Neurology Authors: Solntseva EI, Bukanova JV, Kondratenko RV, Skrebitsky VG Tags: Brain Res Bull Source Type: research