Chemical profiling and identification of anti-osteoporosis chemical-markers of < em > Cinnamomum cassia (L.) < /em > presl extracts using GC-MS and spectrum-activity analyses

Nat Prod Res. 2022 Sep 13:1-5. doi: 10.1080/14786419.2022.2123480. Online ahead of print.ABSTRACTCinnamomum cassia (L.) Presl (cinnamon), an important folk medicine is widely used to prevent osteoporosis for long time in China. Our study aimed to investigate the anti-osteoporosis activity and mechanisms of cinnamon extracts obtained by supercritical CO2 extraction (SFE) and identify activity associated chemical components by gas chromatography-mass spectrometry. The cinnamon SFE exhibited superior anti-osteoporosis efficacy in an ovariectomised mice model to common alcohol extracts. It could induce calcified nodules and ALP activity, upregulate the mRNA expression of ALP, BMP-2, and RUNX2 in MC3T3-E1 cells. The major chemical classes of cinnamon extracts were alcohol esters (28.2%), and terpenes (16.1%). The spectrum-activity analysis indicated that the potential chemical-markers of extracts could be (E)-Cinnamaldehyde, γ-Sitosterol, and (Z, Z)-9,12-Octadecadienoic acid, which could induce the proliferation and ALP activity in MC3T3-E1 cells. Our study revealed the promising applications of the cinnamon SFE in prevention of osteoporosis, and identified its anti-osteoporosis associated compounds.PMID:36098221 | DOI:10.1080/14786419.2022.2123480
Source: Natural Product Research - Category: Biochemistry Authors: Source Type: research