Accurate Classification of Germinal Center B-Cell–Like/Activated B-Cell–Like Diffuse Large B-Cell Lymphoma Using a Simple and Rapid Reverse Transcriptase–Multiplex Ligation-Dependent Probe Amplification Assay A CALYM Study

Publication date: Available online 16 April 2015 Source:The Journal of Molecular Diagnostics Author(s): Sylvain Mareschal , Philippe Ruminy , Cristina Bagacean , Vinciane Marchand , Marie Cornic , Jean-Philippe Jais , Martin Figeac , Jean-Michel Picquenot , Thierry Jo Molina , Thierry Fest , Gilles Salles , Corinne Haioun , Karen Leroy , Hervé Tilly , Fabrice Jardin Diffuse large B-cell lymphoma, the most common non-Hodgkin lymphoma, is subdivided into germinal center B-cell–like and activated B-cell–like subtypes. Unfortunately, these lymphomas are difficult to differentiate in routine diagnosis, impeding the development of treatments. Patients with these lymphomas can benefit from specific therapies. We therefore developed a simple and rapid classifier based on a reverse transcriptase multiplex ligation-dependent probe amplification assay and 14 gene signatures. Compared with the Affymetrix U133+2 gold standard, all 46 samples (95% CI, 92%–100%) of a validation cohort classified by both techniques were attributed to the expected subtype. Similarly, 93% of the 55 samples (95% CI, 82%–98%) of a second independent series characterized with a mid-throughput gene expression profiling method were classified correctly. Unclassifiable sample proportions reached 13.2% and 13.8% in these cohorts, comparable with the frequency originally reported. The developed assay was also sensitive enough to obtain reliable results from formalin-fixed, paraffin-embedded...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research