Sensors, Vol. 22, Pages 6183: Microfluidic-Assisted Human Cancer Cells Culturing Platform for Space Biology Applications

Sensors, Vol. 22, Pages 6183: Microfluidic-Assisted Human Cancer Cells Culturing Platform for Space Biology Applications Sensors doi: 10.3390/s22166183 Authors: Agnieszka Krakos (Podwin) Joanna Jarosz Patrycja Śniadek Mateusz Psurski Adrianna Graja Marcin Białas Ewa Oliszewska Joanna Wietrzyk Rafał Walczak Jan Dziuban In the paper, the lab-on-chip platform applicable for the long-term cultivation of human cancer cells, as a solution meeting the demands of the CubeSat biological missions, is presented. For the first time, the selected cancer cell lines—UM-UC-3 and RT 112 were cultured on-chip for up to 50 days. The investigation was carried out in stationary conditions (without medium microflow) in ambient temperature and utilizing the microflow perfusion system in the incubation chamber assuring typical cultivation atmosphere (37 °C). All the experiments were performed to imitate the conditions that are provided before the biological mission starts (waiting for the rocket launch) and when the actual experiment is initialized on a CubeSat board in space microgravity. The results of the tests showed appropriate performance of the lab-on-chip platform, especially in the context of material and technological biocompatibility. Cultured cells were characterized by adequate morphology—high attachment rate and visible signs of proliferation in each of the experimental stage. These results are a good basis for furthe...
Source: Sensors - Category: Biotechnology Authors: Tags: Article Source Type: research