IL-35 inhibits HBV antigen-specific IFN-{gamma}-producing CTLs in vitro

In this study, we investigatedwhether interleukin-35 is involved in HBV-related cellular immune responses. CD4+ T-cells from peripheral blood were derived from healthy volunteers, resolved HBV individuals and chronic active hepatitis B patients and stimulated with CD3/28-conjugated beads. We analyzed mRNA and protein levels of interleukin-35, and assessed the inhibitory effect of interleukin-35 on HBV core antigen specific cytotoxic T lymphocytes, dendritic cells, and effector T-cells. Correlation analyses between liver inflammation and HBV DNA load were conducted. Results show thatchronic HBV patients harbor significantly higher levels of Epstein-Barr virus-induced gene 3 mRNA and protein in CD4+ T-cells compared to healthy volunteers and resolved HBV individuals. Interleukin-35 suppressed the proliferation of HBVantigen-specific cytotoxic T-lymphocytes and interferon-γ production in vitro. Ex vivo, interleukin-35 decreased the proliferation of CD4+CD45RA+ naïve T-cells, especially in CD4+CD25-CD45RA+ naïve effector T-cells. Interleukin-35 inhibited the expansion of CD11c+ dendritic cells. Ourdata indicate that interleukin-35 is highly expressed in chronic HBV CD4+ T-cells and plays an important role in the inhibition of the cellular immune response in chronic HBV.
Source: Clinical Science - Category: Biomedical Science Authors: Source Type: research

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ConclusionA significant reduction in bone density was observed in patients with chronic hepatitis B receiving Tenofovir for more than 12 months in comparison with the control group. Also, the patients who had been taking Tenofovir in longer time, osteoporosis and osteopenia were more reported.
Source: Clinical Epidemiology and Global Health - Category: Epidemiology Source Type: research
Publication date: Available online 9 October 2019Source: The Lancet Respiratory MedicineAuthor(s): Marcelo Cypel, Jordan J Feld, Marcos Galasso, Rafaela V Pinto Ribeiro, Nikki Marks, Magdalena Kuczynski, Deepali Kumar, Ilona Bahinskaya, Vanderlei S Bagnato, Cristina Kurachi, Arthur S S.sky, Jonathan C Yeung, Laura Donahoe, Marc de Perrot, Kazuhiro Yasufuku, Andrew Pierre, Matthew Binnie, Cecilia Chaparro, Tereza Martinu, Manyin ChenSummaryBackgroundA substantial proportion of organ donors test positive for hepatitis C virus (HCV) infection. To date, only a few studies have evaluated the safety of using lungs from these don...
Source: The Lancet Respiratory Medicine - Category: Respiratory Medicine Source Type: research
CONCLUSIONS The network pharmacology intuitively shows the multi-component, multi-target, and multi-channel pharmacological effects of Paeonia lactiflora on UC, and provides a scientific basis for studying the mechanism of the effect of Paeonia lactiflora on UC. PMID: 31594914 [PubMed - in process]
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
In this study, we explored the interactions between HBV and IFITs (IFN-induced proteins with tetratricopeptide repeats), which are classical IFN-inducible genes. Specifically, we found that HBV patients undergoing IFN-I therapy exhibited elevated expression of IFITs in their peripheral blood mononuclear cells (PBMCs). We further observed upregulation in the expressions of IFIT1, IFIT2, and IFIT3 in cells transfected with the pHBV1.3 plasmid, which yields infectious virions in hepatic cells. We additionally found that HBx, which is the only regulatory protein encoded within the HBV genome, activates NF-κB, which in tu...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
CD8+T cell responses are crucial to prevent and control hepatitis B virus (HBV) infection, but their activity is considered to be abolished during persistent HBV infection. Thus, functional defects in the CD8+T cell responses, termed exhaustion, are considered as the major factor for the chronicity of HBV infection.[1] However, it is unclear how exhausted CD8+T cells are able to mediate the sustained inhibition of HBV replication in some patients with chronic HBV infection. Accumulated data have established that the pool of exhausted CD8+T cells consists of phenotypically and functionally distinct subpopulations.
Source: Journal of Hepatology - Category: Gastroenterology Authors: Source Type: research
Publication date: October 2019Source: European Urology Supplements, Volume 18, Issue 9Author(s): A. Natali, G. Cito, R. Fucci, R. Picone, A. Cocci, M. Sessa, F. Sessa, E. Micelli, F. Rizzello, R. Campi, N. Laruccia, P. Verrienti, L. Criscuoli, S. Serni, M. Carini, M. Coccia
Source: European Urology Supplements - Category: Urology & Nephrology Source Type: research
Publication date: Available online 10 October 2019Source: The Journal of Molecular DiagnosticsAuthor(s): Can Liu, Jinpiao Lin, Zhen Xun, Jinlan Huang, Er Huang, Tianbin Chen, Yujue He, Ni Lin, Bin Yang, Qishui OuDynamic and real-time hepatitis B virus (HBV) DNA, genotype, and reverse transcriptase mutation analysis plays an important role in diagnosing and monitoring chronic hepatitis B (CHB) and in assessing the therapeutic response. We established a highly sensitive coamplification at lower denaturation temperature PCR (COLD-PCR) coupled with probe-based fluorescence melting curve analysis (FMCA) for precision diagnosis ...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research
Authors: Arefkhah N, Vafazadeh S, Shahriarirad S, Ghorbani F, Zoghi S, Emami M, Zamiri B, Cheraghi MR, Sarvari J, Sarkari B Abstract The present study aimed to find out the levels of anti-HBsAb among vaccinated children in a rural community in Fars Province, Southern Iran. Blood samples were taken from 550 children, aged 1-12 years (mean 6.4 ± 3.5), in 2017 from three villages in the area. A structured questionnaire was used to get the sociodemographic data of the subjects along with determinants concerning the Hepatitis B. Sera samples were examined for anti-HBsAb, using an ELISA commercial k...
Source: Journal of Immunoassay and Immunochemistry - Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research
In conclusion, in contrast to previous observations that tumor HBx variants lack transcriptional activity, we showed here that HBx variants have retained their ability to counteract Smc5/6 and thus to activate cccDNA transcription although they tend to lose antiproliferative activity.
Source: Antiviral Therapy - Category: Virology Source Type: research
ConclusionOur study reported the prevalent nature of NSIs among dental assistants in Saudi Arabia and identified key factors that could be targeted to mitigate this preventable condition. Dental assistants would benefit from proper training on infection control protocols and procedures.
Source: Environmental Health and Preventive Medicine - Category: Environmental Health Source Type: research
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