Apoptin interacts with and regulates the activity of protein kinase C beta in cancer cells

Abstract Apoptin, the VP3 protein from chicken anaemia virus (CAV), induces tumour cell-specific cell death and represents a potential future anti-cancer therapeutic. In tumour but not in normal cells, Apoptin is phosphorylated and translocates to the nucleus, enabling its cytotoxic activity. Recently, the β isozyme of protein kinase C (PKCβ) was shown to phosphorylate Apoptin in multiple myeloma cell lines. However, the exact mechanism and nature of interaction between PKCβ and Apoptin remain unclear. Here we investigated the physical and functional link between PKCβ and CAV-Apoptin as well as with the recently identified Apoptin homologue derived from human Gyrovirus (HGyV). In contrast to HCT116 colorectal cancer cells the normal colon mucosa cell lines expressed low levels of PKCβI and showed reduced Apoptin activation, as evident by cytoplasmic localisation, decreased phosphorylation and lack of cytotoxic activity. Co-immunoprecipitation and proximity ligation assay studies identified binding of both CAV- and HGyV-Apoptin to PKCβI in HCT116 cells. Using Apoptin deletion constructs the N-terminal domain of Apoptin was found to be required for interacting with PKCβI. FRET-based PKC activity reporter assays by fluorescence lifetime imaging microscopy showed that expression of Apoptin in cancer cells but not in normal cells triggers a significant increase in PKC activity. Collectively, the results demonstrate a novel cancer specif...
Source: Apoptosis - Category: Molecular Biology Source Type: research

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Worldwide use of pneumococcal conjugate vaccines (PCVs) had reduced the incidence of pneumonia and invasive pneumococcal disease (IPD) in children.1,2 Despite increasing vaccine coverage and a reduction in the prevalence of IPD, Streptococcus pneumoniae remains a significant cause of non-Shiga toxin-producing Escherichia coli hemolytic uremic syndrome (HUS) in children.3 HUS is characterized by the presence of the clinical triad of microangiopathic hemolytic anemia, thrombocytopenia, and acute kidney injury.
Source: Pediatrics and Neonatology - Category: Perinatology & Neonatology Authors: Tags: Editorial Source Type: research
Authors: Bilkhu R, Billè A Abstract The coronavirus 2019 (COVID-19) pandemic has caused significant mortality around the world and the focus has been on reducing the number of infections. In order not to compromise treatment of oncology patients, reducing the number of patients with COVID-19 undergoing treatment is mandatory. We reviewed the experience of the National Institute of Cancer in Milan and compared it with our experience. PMID: 32462984 [PubMed - as supplied by publisher]
Source: Tumori - Category: Cancer & Oncology Tags: Tumori Source Type: research
Conclusion: Adding a BM-TT to FIT-screening considerably reduces colonoscopy burden, but could also decrease screening effectiveness. Combining FIT15 with a high polyp sensitivity BM-TT seems most promising. PMID: 32462913 [PubMed - as supplied by publisher]
Source: Journal of Comparative Effectiveness Research - Category: General Medicine Tags: J Comp Eff Res Source Type: research
This issue of the Surgical Oncology Clinics of North America is devoted to covering the important topic of melanoma. The incidence of primary cutaneous melanoma continues to increase each year. While melanoma accounts for the majority of skin cancer –related deaths, surgical treatment of early disease can be curative. Over the last decade, there have been marked changes in the surgical and systemic treatment of melanoma. For example, within the surgical field, there have been multiple prospective randomized clinical trials to define the exten t of surgery with important changes to how the nodal basin should be managed and staged.
Source: Surgical Oncology Clinics of North America - Category: Surgery Authors: Tags: Foreword Source Type: research
(Nagoya University) Although lymphoma is one of the most common types of blood cancer, it has a rare subtype for which no effective treatment regimens are known. For the first time, Nagoya University researchers and colleagues in Japan have conducted clinical trials for a new treatment protocol and report it to be quite promising.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
PMID: 32460969 [PubMed - in process]
Source: Translational Oncology - Category: Cancer & Oncology Authors: Tags: Transl Oncol Source Type: research
This study investigated the impact of gross extrathyroidal extension into major neck structures on the prognosis of papillary thyroid carcinoma according to changes in the American Joint Committee on Cancer (AJCC)/Tumor-Node-Metastasis staging system. Overall, 183 patients with gross extrathyroidal extension into major neck structures were enrolled. The 10-year disease-specific survival (DSS) of patients in each stage showed appropriate correlation and stratification with the AJCC eighth edition. However, the 10-year DSS rate in stage III was better than the expected 10-year DSS rate, according to the AJCC eighth edition. ...
Source: Endocrine Journal - Category: Endocrinology Tags: Endocr J Source Type: research
In conclusion, although salvage surgery for structural recurrence of PTC has a high risk of complications, it may be worthwhile when macroscopic curative resection is available. The decision should be made considering various factors including curability, risk of surgical procedure, functional outcome, and life expectancy. PMID: 32461508 [PubMed - as supplied by publisher]
Source: Endocrine Journal - Category: Endocrinology Tags: Endocr J Source Type: research
Publication date: Available online 28 May 2020Source: The LancetAuthor(s): Nicole M Kuderer, Toni K Choueiri, Dimpy P Shah, Yu Shyr, Samuel M Rubinstein, Donna R Rivera, Sanjay Shete, Chih-Yuan Hsu, Aakash Desai, Gilberto de Lima Lopes, Petros Grivas, Corrie A Painter, Solange Peters, Michael A Thompson, Ziad Bakouny, Gerald Batist, Tanios Bekaii-Saab, Mehmet A Bilen, Nathaniel Bouganim, Mateo Bover Larroya
Source: The Lancet - Category: General Medicine Source Type: research
Publication date: Available online 28 May 2020Source: The LancetAuthor(s): Lennard Y W Lee, Jean Baptiste Cazier, T Starkey, C D Turnbull, UK Coronavirus Cancer Monitoring Project Team, Rachel Kerr, Gary Middleton
Source: The Lancet - Category: General Medicine Source Type: research
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