UV-CD12: synchrotron radiation circular dichroism beamline at ANKA

Synchrotron radiation circular dichroism (SRCD) is a rapidly growing technique for structure analysis of proteins and other chiral biomaterials. UV-CD12 is a high-flux SRCD beamline installed at the ANKA synchrotron, to which it had been transferred after the closure of the SRS Daresbury. The beamline covers an extended vacuum-UV to near-UV spectral range and has been open for users since October 2011. The current end-station allows for temperature-controlled steady-state SRCD spectroscopy, including routine automated thermal scans of microlitre volumes of water-soluble proteins down to 170 nm. It offers an excellent signal-to-noise ratio over the whole accessible spectral range. The technique of oriented circular dichroism (OCD) was recently implemented for determining the membrane alignment of α-helical peptides and proteins in macroscopically oriented lipid bilayers as mimics of cellular membranes. It offers improved spectral quality

Source: Journal of Synchrotron Radiation - April 11, 2015 Category: Physics Authors: Bürck, J.Roth, S.Windisch, D.Wadhwani, P.Moss, D.Ulrich, A.S. Tags: synchrotron radiation circular dichroism (SRCD) vacuum-UV secondary structure proteins alignment of membrane-active peptides oriented CD (OCD) of anisotropic samples beamlines Source Type: research