Environmental pollutants directly affect the liver X receptor alpha activity: Kinetic and thermodynamic characterization of binding
Publication date: Available online 11 April 2015 Source:The Journal of Steroid Biochemistry and Molecular Biology Author(s): Matteo Mozzicafreddo , Massimiliano Cuccioloni , Laura Bonfili , Valentina Cecarini , Francesco Alessandro Palermo , Paolo Cocci , Gilberto Mosconi , Aida Capone , Irene Ricci , Anna Maria Eleuteri , Mauro Angeletti Liver X receptor is a ligand-activated transcription factor, which is mainly involved in cholesterol homeostasis, bile acid and triglycerides metabolism, and, as recently discovered, in the glucose metabolism by direct regulation of liver glucokinase. Its modulation by exogenous factors, such as drugs, industrial by-products, and chemicals is documented. Owing to the abundance of these synthetic molecules in the environment, and to the established target role of this receptor, a number of representative compounds of phthalate, organophosphate and fibrate classes were tested as ligands/modulators of human liver X receptor, using an integrated approach, combining an in silico molecular docking technique with an optical SPR biosensor binding study. The compounds of interest were predicted and proved to target the oxysterols-binding site of human LXRα with measurable binding kinetic constants and with affinities ranging between 4.3×10−7 and 4.3×10−8 M. Additionally, non-cytotoxic concentration of these chemicals induced relevant changes in the LXRα gene expression levels and other target genes (SREBP-1c and ...
The high saturated fat content of coconut oil could be driving adverse cholesterol changes, authors of a new review of published studies suggest.Medscape Medical News
Contributors : Takuya Nakagawa ; Masaki Fukuyo ; Atsushi KanedaSeries Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensDNA methylation analysis in oropharyngeal squamous carcinoma (OPSCC) samples and oropharyngeal non-cancerous mucosa samples. Infinium MethylationEPIC BeadChip Kit was used to obtain DNA methylation profiles across more than 850,000 CpG sites. Total samples included 89 OPSCC samples and 5 non-cancerous mucosa samples.
Contributors : Takuya Nakagawa ; Masaki Fukuyo ; Atsushi KanedaSeries Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensDNA methylation analysis in oropharyngeal squamous carcinoma (OPSCC) samples and oropharyngeal non-cancerous mucosa samples. Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across 485,577 CpG sites. Total samples included 89 OPSCC samples and 5 non-cancerous mucosa samples.
Contributors : Girieca Lorusso ; Curzio RueggSeries Type : Expression profiling by arrayOrganism : Mus musculusWe recently established an orthotopic breast cancer model of brain metastasis based on the injection of murine breast cancer cell lines into the mammary fat pad. This model is based on the use of 4T1 murine breast carcinoma cells. 4T1-derived tumors recapitulate the main steps of human breast cancer progression, including epithelial-to-mesenchymal transition and metastases to lung and lymph nodes.Bioluminescence imaging revealed the appearance of secondary lesions to the lung and lymph nodes and sporadically to th...
Contributors : Eric Seidel ; Gudrun Walenda ; Ute Scholl ; Liz LiSeries Type : Expression profiling by arrayOrganism : Homo sapiensIn order to investigate gene expression changes in a model of mitotane resistant adrenocortical carcinoma, we generated mitotane resistant clones from HAC15 bulk cells via long-term treatment followed by clonal selection.
Contributor : Gottfrid Sj ödahlSeries Type : Expression profiling by arrayOrganism : Homo sapiensIt is unclear how urothelial carcinoma of the urinary bladder change, genetically and phenotypically, as patients progress from recurrent non muscle-invasive (NMI) to muscle-invasive (MI) disease or disease that require radical cystectomy. This data set contains gene expression data from 200 samples in a longitudinal study of such patients. Overlap with previously GEO-published cohorts from our lab is described in the metadata.
The aim of this retrospective study was to compare the feasibility and efficiency of ultrasound-guided percutaneous microwave ablation (US-PMWA) assisted by three-dimensional visualization ablation planning sy...
Following publication of the original article , the authors reported the errors in Fig. 1C and D, Fig. 2, Fig. 4B and C and Fig. 6D and E.