A simple and rapid method for the quantitation of secreted hepatitis B virions in cell culture models

We report the development of an assay using a commercially available HBsAg-ELISA plate for immunocapture followed by real-time PCR for quantification of hepatitis B virions in cell cultures. This assay is rapid, highly sensitive (50 copies/reaction) and highly specific for virion-associated DNA. In addition, the assay requires only 20 μL of supernatant, allowing scaling down of transfections.
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research